The dilution data of the sheet for your reference.
View Large Extraction and isolation of markers from A. Vacuum concentration yielded hexane extract 32 gCHCl3 extract 20 g and butanol extract 90 g. Butanol extract was subjected to vacuum liquid chromatography over silica gel eluting with a gradient of CHCl3—MeOH each 4. Merck, Darmastadt, Germany was used for optimization of analytical protocol.
Standard and sample solutions were spotted in the form of bands 5 mm10 mm from both the lower and left edge and 10 mm space between two bands, with a microliter syringe using an automatic TLC Sampler, under continuous nitrogen current.
Linear ascending development was carried out in twin trough glass chambers saturated with the mobile phase. The mobile phase selection was carried out using the Vario System wherein different compositions consisting of different ratios of solvents of varying polarity were tried. Finally, a mobile phase consisting of chloroform: The saturation time of the TLC chamber in the mobile phase was optimized to 5 min for a better resolution of the tested markers.
After development, the plates were dried and dipped into vanillin—sulfuric acid reagent vanillin: For recording of characteristic derivatized spot of compounds and sample tracks in the range — nm, a tungsten lamp was used.
Quantitation was performed using area under peak with linear regression of amount in micrograms per band. Photo-documentation after derivatization in white radiation: Statistical analysis Concentration—response curves were obtained using the Prism 5. Working stocks for calibration studies were prepared by dilution using a Hamilton syringe Bonaduz, Switzerland.
Dried and finely powdered plant material mg was extracted with 10 mL using different solvents hexane, chloroform, acetone, ethyl acetate and methanol. Extracted samples were made up to 1 mL and centrifuged at 10, rpm for 5 min prior application on TLC.The crude extract of the stembark of Streblus asper, a traditionally used medicinal plant of India, revealed significant macrofilaricidal activity against Litomosoides carinii and Brugia malayi in rodents.
The study revealed two cardiac glycosides, K (asperoside) and K (strebloside) of the extract to be responsible for antifilarial activity. Diarylheptanoids from Alnus nepalensis leaves have been reported for promising activity against filariasis, a mosquito-borne disease, and this has prompted us to investigate its anti-malarial and safety profile using in-vitro and in-vivo bioassays.
Extraction and isolation of markers from A.
nepalensis. The air-dried powdered leaves of A. nepalensis ( g) were macerated with MeOH (2 L) overnight and evaporated in vacuo to yield crude extract ( g). Soil improver: Produces considerable amounts of litter. Use of the leaf litter as mulch enhances soil fertility however P.
thonningii leaves decompose slowly. Intercropping: A good tree that can be grown with Annona, Grewia and Combretum spp. Competes very little with maize if .
Yunani use the leaves for inflammation, the flowers for a purgative, the fruit as anti-inflammatory, antipyretic, abortifacient, demulcent, purgative, refrigerant, good for chest complaints, eye ailments, flu, heart and liver ailments, and rheumatism, though suspected of inducing asthma.
Alnus nepalensis (D. Don), an alder species, family Betulaceae, is an actinorhizal tree found in the hilly regions of Eastern and Northeastern India.
Diarylheptanoids belong to a phenolic class of natural products based on 1,7-diphenylheptane frame, and many diarylheptanoids have been isolated from Zingiberaceae and Betulaceae plants up to date.